Mouse Endothelin 2 ELISA Kit from Innovative Research is intended for the quantitative determination of Mouse Endothelin 2 in biofluid samples, such as serum, plasma or other biological fluids.This is a sandwich ELISA in strip well format with reagents for up to 96 tests. EDN2 present in samples reacts with an anti-Mouse EDN2 capture antibody coated and dried on a microtiter plate. After appropriate washing steps, an anti-EDN2 detection antibody conjugated to biotin binds to the captured analyte. Excess antibody is washed away and bound antibody is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is directly proportional to the concentration of active EDN2 in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of Mouse EDN2. Detection Target: Mouse Endothelin 2 Reactivity: Mouse Range: 31.2-2000pg/mL Sensitivity: 11.2pg/mL Storage Conditions: Store all components at 4┬░C This Mouse EDN2 activity assay is for the quantitative determination of Endothelin 2 in samples including serum, plasma or other biological fluid For research use only. Strip well format. Reagents for up to 96 tests. For research use only. At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.